Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-disease-resistance tissue culture seedling raising method for baby cabbages

A technology of baby cabbage group and disease resistance, which is applied in gardening methods, botanical equipment and methods, gardening and other directions, and can solve the problems of soil compaction, affecting the quality and yield of baby cabbage, and low fertilizer utilization rate.

Inactive Publication Date: 2021-05-18
云南帅豪农牧科技有限公司
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to local climatic conditions, farmers apply fertilizers blindly, resulting in frequent occurrence of physiological problems in baby vegetables, which affect the quality and yield of baby vegetables; and farmers apply fertilizers blindly, resulting in low fertilizer utilization efficiency and serious soil compaction

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1) Take the material, take the well-grown baby cabbage leaves and wash them with running water for 30-60 minutes, dry them with filter paper, disinfect them with 85% alcohol for 2-3 seconds, perform detoxification treatment, wash them with distilled water, and cut them into (1.0-1.4)cm×(1.0- 1.4) cm small square;

[0055] 2) Cultivate, move the processed baby cabbage tissue into the basic culture medium, spray the infected bacteria liquid, spray twice with an interval of 24h, spray 0.5-0.8ml for the first time, spray 2-3ml for the second time, cultivate in the culture room, Cultivate in a dark room for 20-25 days at a room temperature of 15-20°C to produce callus;

[0056] 3) Differentiation, cut the callus into pieces, inoculate it on the differentiation medium, and culture it for 20-25 days under the conditions of light 12h / d, light intensity 2600Lx, room temperature 20-22°C, and induce callus differentiation form adventitious buds;

[0057] 4) Rooting and strong se...

Embodiment 2

[0075] 1) Take the material, take the well-grown baby cabbage leaves and wash them with running water for 30-60 minutes, dry them with filter paper, disinfect them with 85% alcohol for 2-3 seconds, perform detoxification treatment, wash them with distilled water, and cut them into (1.0-1.4)cm×(1.0- 1.4) cm small square;

[0076] 2) Cultivate, move the processed baby cabbage tissue into the basic culture medium, spray the infected bacteria liquid, spray twice with an interval of 24h, spray 0.5-0.8ml for the first time, spray 2-3ml for the second time, cultivate in the culture room, Cultivate in a dark room for 20-25 days at a room temperature of 15-20°C to produce callus;

[0077] 3) Differentiation, cut the callus into pieces, inoculate it on the differentiation medium, and culture it for 20-25 days under the conditions of light 12h / d, light intensity 2600Lx, room temperature 20-22°C, and induce callus differentiation form adventitious buds;

[0078] 4) Rooting and strong se...

Embodiment 3

[0096] 1) Take materials, take well-grown Chinese baby cabbage leaves, wash them with running water for 40 minutes, blot them dry with filter paper, sterilize them with 85% alcohol for 2-3 seconds, perform detoxification treatment, wash them with distilled water, and cut them into (1.0-1.4)cm×(1.0-1.4) cm small square;

[0097] 2) Cultivate, move the processed baby cabbage tissue into the basic medium, spray the infection bacteria liquid, spray twice, with an interval of 24h, spray 0.6ml for the first time, spray 2ml for the second time, cultivate in a culture room, and cultivate in a dark room 20~25d, room temperature 15~20℃, produce callus;

[0098] 3) Differentiation, cut the callus into pieces, inoculate it on the differentiation medium, and culture it for 20-25 days under the conditions of light 12h / d, light intensity 2600Lx, room temperature 20-22°C, and induce callus differentiation form adventitious buds;

[0099] 4) Rooting and strong seedling cultivation, the adven...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a high-disease-resistance tissue culture seedling raising method for baby cabbages. The high-disease-resistance tissue culture seedling raising method comprises the following steps: (1) material taking, (2) cultivation, (3) differentiation, (4) rooting and strong seedling culture, (5) multiplication culture, (6) rooting culture and (7) temporary planting: exercising rooted test-tube seedlings in a greenhouse for 5-7 d, washing a culture medium left on roots with water, and temporarily planting the seedlings in a rhizoid substrate; and after 15-20 days, new roots grow out and baby cabbagerooting seedlings are obtained. The high-disease-resistance tissue culture seedling raising method improves disease resistance of baby cabbages, can effectively resist infection of the baby cabbages by pathogenic microorganisms, enhances a disease resistance advantage of the baby cabbages, effectively increases a planting survival rate and improves economic benefits.

Description

technical field [0001] The invention relates to the technical field of planting, in particular to a method for cultivating baby cabbage group seedlings with high disease resistance. Background technique [0002] Baby cabbage is a small pocket cabbage, which belongs to the Brassica cabbage subspecies of Brassicaceae. The general growth period is 45-55 days, the commercial ball is 20 cm high, 8-9 cm in diameter, and the net vegetable weighs about 150-200 grams. The suitable temperature for growth is 5°C to 25°C. If it is lower than 5°C, it will be susceptible to freezing damage, making the balls loose or unable to hold the balls; if it is higher than 25°C, it will be susceptible to viral diseases. Baby cabbage is thin, sweet and tender, and delicious. However, due to local climatic conditions, farmers apply fertilizers blindly, resulting in frequent occurrence of physiological problems in baby vegetables, which affect the quality and yield of baby vegetables; and farmers app...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 张远东杨红芬
Owner 云南帅豪农牧科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com