Protein polypeptide drug long-acting microspheres and preparation method thereof
A protein polypeptide and polypeptide technology, which is applied in pharmaceutical formulations, peptide/protein components, medical preparations with non-active ingredients, etc. problem, to achieve the effect of stable and long-lasting drug release rate, good reproducibility and low burst release rate
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Embodiment 1
[0052] Embodiment 1: Preparation of bovine serum albumin microspheres
[0053] The preparation method of the bovine serum albumin microspheres of the present embodiment may further comprise the steps:
[0054] (1) Bovine serum albumin is dissolved in the aqueous solution that contains 20mg / mL mannitol, obtains the aqueous phase (W) that contains bovine serum albumin concentration and is 20mg / mL, the polylactic acid-glycolic acid copolymer ( PLGA, LA:GA=50:50, molecular weight 40000Da) was dissolved in a mixed solvent of dichloromethane and dimethyl carbonate 2:1 (v:v) to obtain an oil phase (O) with a polymer concentration of 50mg / mL ;
[0055] (2) Add the prepared water phase (bovine serum albumin aqueous solution) into the oil phase (PLGA organic solution) (the volume ratio of oil to water is 20:1), and use a cell disruptor in an ice bath with a power of 400W and a duration of 30s. Under the conditions, the two are emulsified to prepare W / O emulsion;
[0056] (3) The abov...
Embodiment 2
[0060] Embodiment 2: Preparation of bovine serum albumin microspheres
[0061] The preparation method of the bovine serum albumin microspheres of the present embodiment may further comprise the steps:
[0062] (1) Dissolve bovine serum albumin in an aqueous solution containing 20 mg / mL mannitol to obtain a water phase (W) containing bovine serum albumin at a concentration of 20 mg / mL. Polylactic acid-glycolic acid copolymer (PLGA , molecular weight 20000Da, LA:GA=50:50) is dissolved in the mixed solvent of dichloromethane and dimethyl carbonate 2:1 (v:v), obtains the oily phase (O) that polymer concentration is 50mg / mL;
[0063] (2) Add the prepared water phase (bovine serum albumin aqueous solution) into the oil phase (PLGA organic solution) (the volume ratio of oil to water is 20:1), and use a cell disruptor in an ice bath with a power of 400W and a duration of 30s. Under the conditions, the two are emulsified to prepare W / O emulsion;
[0064] (3) The above-mentioned W / O e...
Embodiment 3
[0066] Embodiment 3: Preparation of salmon calcitonin microspheres
[0067] The preparation method of the salmon calcitonin microspheres of the present embodiment comprises the steps:
[0068] (1) Dissolve salmon calcitonin in an aqueous solution containing 20 mg / mL mannitol to obtain a water phase (W) containing salmon calcitonin at a concentration of 20 mg / mL, polylactic acid-glycolic acid copolymer (PLGA, molecular weight 40000Da) was dissolved in a mixed solvent of dichloromethane and dimethyl carbonate 2:1 (v:v), to obtain an oil phase (O) with a polymer concentration of 50 mg / mL;
[0069] (2) Add the prepared water phase (salmon calcitonin aqueous solution) into the oil phase (addition of PLGA organic solution) (the volume ratio of oil to water is 20:1), and use the cell disruptor in an ice bath with a power of 400W and a duration of 30s Under certain conditions, the two are emulsified to prepare a W / O emulsion;
[0070] (3) The above-mentioned W / O emulsion is supplied...
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