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Ginseng extract containing endogenous polypeptides and preparation and determinaiton thereof, medicine or medicine composition containing ginseng extract and application of medicine or medicine composition

A ginseng extraction, endogenous technology, applied in the field of natural medicine, can solve problems such as memory loss and work efficiency, and achieve the effect of reducing dosage, promoting smooth transformation and high content

Active Publication Date: 2017-12-29
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the rapid development of modern social economy, people are under increasing pressure in study and work, which has become a health problem that cannot be ignored. It can cause anxiety, insomnia, depression, etc., and eventually lead to memory loss and work efficiency.

Method used

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  • Ginseng extract containing endogenous polypeptides and preparation and determinaiton thereof, medicine or medicine composition containing ginseng extract and application of medicine or medicine composition
  • Ginseng extract containing endogenous polypeptides and preparation and determinaiton thereof, medicine or medicine composition containing ginseng extract and application of medicine or medicine composition
  • Ginseng extract containing endogenous polypeptides and preparation and determinaiton thereof, medicine or medicine composition containing ginseng extract and application of medicine or medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Solvent extraction: ginseng powder was soaked in 40% methanol for 1 hour, ultrasonically extracted for 30 minutes, and the supernatant was collected after centrifugation;

[0040] 2) C18 column separation: the above supernatant was concentrated under reduced pressure to 5% of the original volume and then separated by a C18 column, and then 10% (v / v), 30% (v / v), 40% (v / v), 50% (v / v), 70% (v / v), 90% (v / v) acetonitrile elution, flow rate 0.5mL / min, collect 40% acetonitrile eluate, concentrate and dry to obtain Ginseng extract;

[0041] 3) Content determination: UHPLC-Q-TOF was used to detect the content of endogenous polypeptides in the above extracts. Chromatographic column: Agilent Prorpshell 120 SB C18 2.7μm 3.0×150mm, all in volume fraction, mobile phase A: 0.5% formic acid, mobile Phase B: acetonitrile, gradient elution: 0-15min, 5%-90% B, ion source: ESI source, detection mode: positive ion; use Progenesis QI software to perform peak identification, peak filterin...

Embodiment 2

[0043] 1) Solvent extraction: ginseng powder was soaked in 10 times the volume of 60% ethanol for 1 hour, ultrasonically extracted for 30 minutes, and the supernatant was collected after centrifugation;

[0044] 2) HPD100 resin column separation: the above supernatant was concentrated under reduced pressure to 5% of the original volume and then separated by HPD100 resin column. % (v / v), 70% (v / v), 90% (v / v) ethanol elution, flow rate 0.5mL / min, collect 50% ethanol eluate, concentrate and dry to obtain ginseng extract;

[0045] The endogenous polypeptide content was detected by UHPLC-Q-TOF to be 30%.

Embodiment 3

[0047] 1) Solvent extraction: ginseng powder was soaked with 10 times the volume of 80% formic acid for 1 hour, ultrasonically extracted in an ice bath for 30 minutes, and the supernatant was collected after centrifugation;

[0048] 2) MCI column separation: after the above supernatant was concentrated under reduced pressure to 5% of the original volume, it was separated by MCI (CHP20 / P120) column, and the acid was eluted with 10 times column volume of water, and then 2 times column volume 10 %(v / v), 30%(v / v), 40%(v / v), 50%(v / v), 70%(v / v), 90%(v / v) and 100%( v / v) methanol elution, flow rate 0.5mL / min, collect 40% methanol eluate, concentrate and dry to obtain ginseng extract;

[0049] The content of endogenous polypeptide detected by UHPLC-Q-TOF was 33%.

[0050] Examples of Identification of Ginseng Endogenous Polypeptides

[0051] Example 1

[0052] 1) Analysis: The ginseng extract obtained in "Ginseng Extract Preparation Example 1" was dissolved with 0.1% (v / v) formic ac...

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Abstract

The invention provides a preparation method of a ginseng extract containing endogenous polypeptides, the ginseng extract, a determination method of the polypeptides in the ginseng extract, a medicine or medicine composition containing the ginseng extract and an application of the medicine or medicine composition. The preparation method comprises the steps of grinding a ginseng medicinal material into powder, extracting by an organic solvent or formic acid, separating the extract liquid by a column, concentrating the eluent, and thus obtaining the ginseng extract with the endogenous ginseng polypeptides as a main component. The determination method of the ginseng extract comprises the steps that a Nano LC-LTQ-Orbitrap technology is used for analysis, a Swiss-Prot database is searched by PEAKS, 308 endogenous polypeptides are identified from the ginseng extract totally, amino acid sequences of the polypeptides are SEQ ID No.1-125 and SEQ ID NoS.1-183, and the molecular weight is ranged from 300 to 7000 Da. Through UHPLC-Q-TOF detection, the content of the ginseng polypeptides of the extract is more than 30% by ProgenesisQI software analysis. The ginseng extract is also subjected to cell experiments, and experimental results indicate that the extract has a function of promoting the proliferation of PC 12 cells.

Description

technical field [0001] The invention belongs to the field of natural medicines. It specifically relates to a preparation method of ginseng extract containing endogenous polypeptides, a ginseng extract, a method for determining polypeptides in ginseng extracts, a medicine or a pharmaceutical composition containing ginseng extracts, and applications thereof, and the polypeptides are derived from ginseng Extract and endogenous polypeptides. Background technique [0002] With the rapid development of modern social economy, people are under increasing pressure in study and work, which has become a health problem that cannot be ignored. It can cause anxiety, insomnia, depression, etc., and eventually lead to memory loss and work efficiency. In addition, under normal physiological conditions, with age and body aging, learning and memory abilities will also decline. Therefore, it is necessary to develop health products that have neuroprotective effects or improve learning and memo...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P25/00A61P25/28G01N1/28C07K7/06C07K7/08C07K14/415C07K1/20C07K1/22C12N5/09
CPCA61K36/258A61K2236/15A61K2236/333A61K2236/55C07K7/06C07K7/08C07K14/415C12N5/0693C12N2500/76G01N1/28
Inventor 张晓哲刘欣欣程孟春赵楠
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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