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A method for the treatment of pancreatic cancer based on gold nanoclusters combined with ngf siRNA

A technology of gold nanoclusters and nanoclusters, which is applied in the direction of DNA/RNA fragments, gene therapy, recombinant DNA technology, etc., to achieve the effects of improving stability, improving anti-tumor treatment effect, and simple preparation method

Active Publication Date: 2022-07-26
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, free siRNA is easily degraded by nucleases in serum. Therefore, it is necessary to further develop pharmaceutical compositions and preparations based on NGF siRNA with better stability for preventing and / or treating cancer.

Method used

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  • A method for the treatment of pancreatic cancer based on gold nanoclusters combined with ngf siRNA
  • A method for the treatment of pancreatic cancer based on gold nanoclusters combined with ngf siRNA
  • A method for the treatment of pancreatic cancer based on gold nanoclusters combined with ngf siRNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] This example is used to illustrate the NGF siRNA combined with gold nanoclusters (GNC-siRNA) of the present invention and the preparation method thereof.

[0074] The GNC-siRNA of the present invention is prepared by the following steps (technical routes such as figure 1 shown):

[0075] (1) Add HAuCl to HAuCl at 25°C 4 The solution was added with reducing agents GSH and CR 9 , where HAuCl 4 100mM, 150mM GSH, CR 9 75mM, equivalent to HAuCl 4 The molar ratio to GSH is 1:1.5, HAuCl 4 with CR 9 The molar ratio of 1:0.75 was then heated to 70 °C and stirred at constant temperature for 24 h to obtain a gold nanocluster solution.

[0076] The color of the gold nanocluster solution is light yellow-green (e.g. figure 2 shown. Due to patent publication, figure 2 Incorrect colors may be displayed, which does not limit the invention). The size and surface morphology of gold nanoclusters were observed by TEM, as shown in the TEM image. image 3 As shown, its average d...

Embodiment 2～10

[0080] This example is used to illustrate the GNC-siRNA of the present invention and its preparation method.

[0081]The GNC-siRNAs of Examples 2 to 10 were prepared in the same manner as in Example 1, except that the mass ratios of NGF siRNA and gold nanoclusters in step (2) were 0.5:1, 1:1, and 1.5:1, respectively. 1, 2:1, 2.5:1, 3.5:1, 4:1, 5:1, 100:1.

[0082] The zeta potential of the above GNC-siRNA was measured using a zeta potentiometer, and the results are shown in Figure 5 . Wherein, the gold nanocluster solution of step (2) is taken as the mass ratio of 0:1. from Figure 5 It can be seen that when the mass ratio of NGF siRNA / GNC is greater than 3, the zeta potential of the solution does not change, indicating that the siRNA reaches a saturated state. Therefore, the siRNA loading of GNC-siRNA can reach up to 3000 mgsiRNA / g gold nanoclusters, which is equivalent to 226 μmol siRNA / g gold nanoclusters.

[0083] It can be seen that in the GNC-siRNA obtained in Exam...

Embodiment 11

[0085] This example is used to illustrate the GNC-siRNA of the present invention and its preparation method.

[0086] The GNC-siRNA of the present invention is prepared by the following steps (technical routes such as figure 1 shown):

[0087] (1) Add HAuCl to HAuCl at 24°C 4 The solution was added with reducing agents GSH and CR 9 , where HAuCl 4 100mM, 50mM GSH, CR 9 50mM, equivalent to HAuCl 4 The molar ratio to GSH is 1:0.5, HAuCl 4 with CR 9 The molar ratio is 1:0.5. Then heated to 70 °C and stirred at constant temperature for 12 h to obtain a gold nanocluster solution. The color of the gold nanocluster solution was light yellow-green. The size and surface morphology of the gold nanoclusters were observed by TEM, with an average diameter of 2.41 nm and good dispersion. The surface of the gold nanoclusters was positively charged using a zeta potential meter, and the zeta potential was 20.3 mV.

[0088] (3) NGF siRNA was added to the gold nanocluster solution, an...

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Abstract

The present invention provides an NGF siRNA combined with gold nanoclusters, wherein the NGF siRNA combined with gold nanoclusters comprises gold nanoclusters and NGF siRNA, and the gold nanoclusters and the NGF siRNA are combined by electrostatic adsorption. The present invention also provides a preparation method of the NGF siRNA in combination with gold nanoclusters, a pharmaceutical composition and a kit containing the NGF siRNA in combination with gold nanoclusters, and the NGF siRNA in combination with gold nanoclusters in the preparation of medicines for preventing and / or treating cancer. application in medicine. The NGF siRNA combined with the gold nanoclusters of the present invention can improve the stability of the siRNA, prolong the circulation time in the body, improve the targeting of pancreatic tumors, improve the silencing effect of NGF, and improve the anti-tumor therapeutic effect of pancreatic cancer, and the preparation method is simple and reactive The mild conditions have opened up new avenues for the treatment of pancreatic cancer.

Description

technical field [0001] The invention relates to a gold nano-cluster, in particular to a combination of NGF siRNA and gold nano-clusters, a pharmaceutical composition and kit containing the NGF siRNA and the combination of gold nano-clusters, and a preparation method and application of the NGF siRNA and the combination of gold nano-clusters. Background technique [0002] Gold nanoclusters (GNCs) have remarkable Coulomb blocking effect and special magnetic properties, and the electronic structure similar to that of semiconductors also makes them have special fluorescence properties, so they are used in biomedical fields for bioimaging and biomolecules (DNA, protein, enzyme) detection, etc. The reported applications of gold nanoclusters are, for example: gold nanoclusters modified with TAT polypeptides for fluorescence imaging, gene delivery, and photodynamic therapy excited by near-infrared light; gold nanoclusters encapsulated with polyacrylic acid and calcium phosphate to pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K9/51A61K31/713A61K47/02A61P35/00A61K33/242
CPCA61K9/5115A61K31/713A61K33/242A61K45/00A61K9/14C12N15/11C12N15/63
Inventor 蒋兴宇雷祎凤谢阳州昀
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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