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Biomarker for detecting ischemic cerebral stroke and application thereof

A biomarker technology for ischemic stroke, applied in the field of biomedicine, can solve problems such as lack of biomarkers

Active Publication Date: 2016-11-23
北京致成生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biomarkers in cerebrovascular disease are still relatively lacking

Method used

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  • Biomarker for detecting ischemic cerebral stroke and application thereof
  • Biomarker for detecting ischemic cerebral stroke and application thereof
  • Biomarker for detecting ischemic cerebral stroke and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The collection of embodiment 1 sample

[0055] From June 2015 to December 2015, patients with ischemic stroke who were treated in PLA Rocket Army General Hospital were collected. A total of 6 cases were collected in the case group. A total of 6 healthy volunteers were collected, and the specific information is shown in Table 1. Obtain 5ml of cubital median venous blood from all research subjects, put it in a 4-degree refrigerator for 1 hour, centrifuge at 1500 rpm for 10 minutes, take the supernatant, centrifuge at 1500 rpm for 10 minutes, take the supernatant, and store in a -80-degree refrigerator.

[0056] Table 1 Sample information

[0057]

[0058]

Embodiment 2

[0059] Example 2 iTRAQ experiment screening differential proteins

[0060] 1. Instruments and reagents used

[0061] Vortex oscillator (Haimen Qilinbeier Instrument Manufacturing Co., Ltd., model: QL-901)

[0062] Constant temperature incubator (Shanghai Pudong Rongfeng Scientific Instrument Co., Ltd., model: HH.S4)

[0063] Vacuum freeze dryer (Thermo, model: SPD2010-230 )

[0064] RIGOL L-3000 HPLC system (Beijing Puyuan Jingdian Technology Co., Ltd.), mobile phase A: 98% ddH2O, 2% acetonitrile (pH 10); mobile phase B: 98% acetonitrile, 2% ddH 2O (pH 10) high performance liquid chromatography: (ThermoScientic EASY-nLC 1000System (Nano HPLC)), mobile phase A: 100% ultrapure water, 0.1% formic acid; mobile phase B: 100% acetonitrile, 0.1% formic acid

[0065] Mass spectrometry system (Thermo, model: Q-Exactive )

[0066] Blue Albumin

[0067] IgG Removal Kit (Sigma-Aldrich, Cat. No.: PROTBA-1KT )

[0068] DTT (Bio-Rad, catalog number: 161-0611, USA )

[0069] Iodoacet...

Embodiment 3

[0136] Example 3 Large sample verification of expression of differential protein KRT9

[0137] 1. Sampling

[0138] From January 2014 to December 2015, patients with ischemic stroke who were treated in the PLA Rocket Army General Hospital were collected. A total of 20 cases were collected from the case group, labeled A1-A20, and the controls were from the PLA Rocket Army General Hospital during the same period. A total of 10 healthy volunteers whose physical examinations were normal but whose indicators were normal were collected and labeled B1-B10. Obtain 5ml of cubital median venous blood from all research subjects, put it in a 4-degree refrigerator for 1 hour, centrifuge at 1500 rpm for 10 minutes, take the supernatant, centrifuge at 1500 rpm for 10 minutes, take the supernatant, and store in a -80-degree refrigerator.

[0139] 2. Experimental process

[0140] The experiment uses human keratin, type I cytoskeleton protein 9 (KRT9) ELISA kit (purchased from Xiamen Huijia B...

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Abstract

The invention discloses a biomarker for detecting the ischemic cerebral stroke. The biomarker comprises KRT9 protein or active fragments thereof, mRNA coding the KRT9 protein, and a gene coding the KRT9 protein. The invention further discloses the KRT9 protein related to the cerebral stroke. Up-regulated expression of the KRT9 protein or the active fragments thereof in cerebral stroke tissue is further verified. By detecting the ischemic cerebral stroke with the protein, early detection can be rapidly and effectively achieved, and a therapeutic target and an important basis are provided for clinical application such as gene therapy and drug therapy.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a biomarker for detecting ischemic stroke and its application. Background technique [0002] Stroke is also called "stroke" or "cerebrovascular accident" (cerebral vascular accident, CVA). It is an acute cerebrovascular disease, which is a group of diseases that cause brain tissue damage due to the sudden rupture of blood vessels in the brain or the inability of blood to flow into the brain due to vascular obstruction, including ischemic and hemorrhagic stroke. [0003] Ischemic stroke, also known as cerebral infarction, is called stroke in traditional Chinese medicine. The disease is caused by a variety of causes of blood supply disturbance in the local brain tissue, leading to ischemic and hypoxic lesions and necrosis of the brain tissue, resulting in clinically corresponding neurological deficits. Ischemic stroke is divided into main types such as cerebral thrombosis, cerebral emb...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893
Inventor 李永旺麻莉康艳娜马玉恒张树峰韩月鹏
Owner 北京致成生物医学科技有限公司
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