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Application of PCYOX1 (Prenylcysteine oxidase 1) protein as biomarker of ischemic cerebral stroke

Active Publication Date: 2016-11-09
北京致成生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biomarkers in cerebrovascular disease are still relatively lacking

Method used

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  • Application of PCYOX1 (Prenylcysteine oxidase 1) protein as biomarker of ischemic cerebral stroke
  • Application of PCYOX1 (Prenylcysteine oxidase 1) protein as biomarker of ischemic cerebral stroke
  • Application of PCYOX1 (Prenylcysteine oxidase 1) protein as biomarker of ischemic cerebral stroke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The collection of embodiment 1 sample

[0057] From June 2015 to December 2015, patients with ischemic stroke who were treated in PLA Rocket Army General Hospital were collected. A total of 6 cases were collected in the case group. A total of 6 healthy volunteers were collected, and the specific information is shown in Table 1. Obtain 5ml of cubital median venous blood from all research subjects, put it in a 4-degree refrigerator for 1 hour, centrifuge at 1500 rpm for 10 minutes, take the supernatant, centrifuge at 1500 rpm for 10 minutes, take the supernatant, and store in a -80-degree refrigerator.

[0058] Table 1 Sample Information

[0059] factor

Embodiment 2

[0060] Example 2 iTRAQ experiment screening differential proteins

[0061] 1. Instruments and reagents used

[0062] Vortex oscillator (Haimen Qilinbeier Instrument Manufacturing Co., Ltd., model: QL-901)

[0063] Constant temperature incubator (Shanghai Pudong Rongfeng Scientific Instrument Co., Ltd., model: HH.S4)

[0064] Vacuum freeze dryer (Thermo, model: SPD2010-230 )

[0065]RIGOL L-3000 HPLC system (Beijing Puyuan Jingdian Technology Co., Ltd.), mobile phase A: 98% ddH2O, 2% acetonitrile (pH 10); mobile phase B: 98% acetonitrile, 2% ddH 2 O (pH 10) high performance liquid chromatography: (ThermoScientic EASY-nLC 1000 System (Nano HPLC)), mobile phase A: 100% ultrapure water, 0.1% formic acid; mobile phase B: 100% acetonitrile, 0.1% formic acid

[0066] Mass spectrometry system (Thermo, model: Q-Exactive )

[0067] Blue Albumin

[0068] IgG Removal Kit (Sigma-Aldrich, Cat. No.: PROTBA-1KT )

[0069] DTT (Bio-Rad, catalog number: 161-0611, USA )

[0070] Iodoace...

Embodiment 3

[0138] Example 3 Large sample verification of expression of differential protein PCYOX1

[0139] 1. Sampling

[0140] From June 2015 to December 2015, patients with ischemic stroke who were treated in the PLA Rocket Army General Hospital were collected. A total of 20 cases were collected from the case group, labeled A1-A20. The controls were from the PLA Rocket Army General Hospital during the same period. A total of 10 healthy volunteers whose physical examinations were normal but whose indicators were normal were collected and labeled B1-B10. Obtain 5ml of cubital median venous blood from all research subjects, put it in a 4-degree refrigerator for 1 hour, centrifuge at 1500 rpm for 10 minutes, take the supernatant, centrifuge at 1500 rpm for 10 minutes, take the supernatant, and store in a -80-degree refrigerator.

[0141] 2. Experimental process

[0142] The experiment used human isopentenyl cysteine ​​oxidase 1 (PCYOX1) ELISA kit (purchased from Shanghai Kanglang Biotec...

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Abstract

The invention discloses an application of PCYOX1 (Prenylcysteine oxidase 1) protein as a biomarker of ischemic cerebral stroke, and particularly discloses the application of the PCYOX1 protein, an active fragment of the PCYOX1 protein or a substance for detecting the PCYOX1 protein or the active fragment of the PCYOX1 protein in a product for diagnosing ischemic cerebral stroke and / or evaluating the risk of suffering from ischemic cerebral stroke. The PCYOX1 protein related with cerebral stroke is provided, and down-regulated expression of the PCYOX1 protein or the active fragment of the PCYOX1 protein in the cerebral stroke tissue is further proved. With the adoption of the protein for detecting cerebral stroke, not only can early detection be realized rapidly and effectively, but also therapeutic targets and important basis are provided for clinical applications such as gene therapy, drug therapy and the like.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of PCYOX1 protein as a biomarker of ischemic stroke. Background technique [0002] Stroke is also called "stroke" or "cerebrovascular accident" (cerebral vascular accident, CVA). It is an acute cerebrovascular disease, which is a group of diseases that cause brain tissue damage due to the sudden rupture of blood vessels in the brain or the inability of blood to flow into the brain due to vascular obstruction, including ischemic and hemorrhagic stroke. [0003] Ischemic stroke, also known as cerebral infarction, is called stroke in traditional Chinese medicine. The disease is caused by a variety of causes of blood supply disturbance in the local brain tissue, leading to ischemic and hypoxic lesions and necrosis of the brain tissue, resulting in clinically corresponding neurological deficits. Ischemic stroke is divided into main types such as cerebral thrombosis, cerebr...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68G01N33/573A61K45/00A61P9/10
CPCA61K45/00C12Q1/6883C12Q2600/118C12Q2600/136C12Q2600/158G01N33/573G01N33/6893G01N2333/90212G01N2800/2871
Inventor 李永旺康艳娜麻莉马玉恒张树峰韩月鹏
Owner 北京致成生物医学科技有限公司
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