A Multicolor Fluorescence Imaging System Based on Supercontinuum Light Source

Abstract

The invention provides a super-continuum spectrum light source based multicolor fluorescence imaging system. The system comprises a super-continuum spectrum laser generating device, a laser wavelength selecting device and an imaging device which are coupled in turn, wherein the super-continuum spectrum laser generating device is used for emitting super-continuum spectrum laser beams to the laser wavelength selecting device; the laser wavelength selecting device is used for successively selecting the single-wavelength laser beams in different wavelengths from the acquired super-continuum spectrum laser beams according to a preset rule; and the imaging device is used for acquiring a plurality of monochrome fluorescent light emitted by a to-be-detected object after being motivated by a plurality of single-wavelength laser beams in turn, generating a plurality of monochrome fluorescent images according to the acquired monochrome fluorescent light and stacking the monochrome fluorescent images, thereby acquiring a multicolor fluorescent image of the to-be-detected object. The irradiated frequency and time of different fluorogens in the to-be-detected object can be reduced and photo-thermal damage and light bleaching can be effectively reduced.

Description

technical field [0001] The invention relates to the field of fluorescence imaging, in particular to a multicolor fluorescence imaging system based on a supercontinuum light source. Background technique [0002] Optical imaging based on fluorescent labeling can specifically label different fluorophores (including fluorescent proteins, dyes, etc.) into the interior of the analyte. These fluorophores are capable of long-term stable existence and reproducible observation in the analyte. [0003] However, multi-color fluorescence-labeled analyte imaging detection analyte deep tissue imaging still faces certain difficulties. On the one hand, since the excitation light will experience strong scattering and absorption when penetrating the tissue, the imaging depth of the imaging system must be large enough; on the other hand, since the optimal excitation wavelengths of multicolor fluorophores are different, so , the power spectral density of a broad-spectrum light source is requir...

AI Technical Summary

Problems solved by technology

However, these light sources have some disadvantages for multicolor fluorescence imaging: the spectrum of hot incandescent lamps (such as tungsten-halogen lamps and xenon lamps) can almost cover the excitation peaks of all fluorophores, but their service life is limited and high-power continuous light irradiation may cause Photothermal damage and photobleaching make it impossible to achieve high-brightness multicolor fluorescence excitation; while LEDs and solid-state lasers have limited spectral linewidths, it is also difficult to achieve multicolor fluorescence excitation

However, these light sources have some disadvantages for multicolor fluorescence imaging: the spectrum of hot incandescent lamps (such as tungsten-halogen lamps and xenon lamps) can almost cover the excitation peaks of all fluorophores, but their service life is limited and high-power continuous light irradiation may cause Photothermal damage and photobleaching make it impossible to achieve high-brightness multicolor fluorescence excitation; while LEDs and solid-state lasers have limited spectral linewidths, it is also difficult to achieve multicolor fluorescence excitation

Therefore, fluorescence imaging is limited by existing excitation light sources, and there are certain difficulties in the study of multicolor labeling in deep tissues.

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