A three-dimensional staining method for cell sections of Korla fragrant pear fruit

Abstract

The invention provides a stereoscopic dyeing method for a cell slide of a Korla pear. The stereoscopic dyeing method is characterized by mixing a hematoxylin absolute ethyl alcohol solution with a concentration of 5%, a ferric trichloride solution with a concentration of 10%, an iodine-potassium iodide solution and a hydrogen peroxide solution with a concentration of 30% in a certain ratio and then carrying out dyeing on the cell slide of the Korla pear, wherein the cell walls are dyed brown, cell nucleuses are dyed atropurpureus, sclereids are dyed light yellow to brown, transfusion tissue is dyed light brown, and cells at each part are clear, visible and distinct, so stereoscopic dyeing effect is achieved. The stereoscopic dyeing method provided by the invention overcomes the defects that dyeing time is difficult to control, sclereids and transfusion tissue are indistinct, and boundaries of pulp cells are not clear in the prior art. The stereoscopic dyeing method is applicable to research on cells in each stable of growth and development of the Korla pear. The stereoscopic dyeing method is simple to operate and saves time and materials; and the prepared cell slide can be observed without a cover slip and a permanent slide for scientific research and teaching can be prepared from the cell slide after treatment with xylene.

Description

technical field [0001] This patent has invented a method for three-dimensional dyeing of Korla fragrant pear fruit slices, which is characterized in that 5% hematoxylin anhydrous ethanol solution, 10% ferric chloride solution, iodine-potassium iodide solution and 30% hydrogen peroxide solution Stain the Korla fragrant pear fruit slices processed by conventional paraffin section method after mixing, observe the growth and changes of epidermal cells, pulp cells, stone cells and conducting tissue cells, and study the development process of various cells in the Korla fragrant pear fruit. Provide technical support for the screening of fertilization formula and the selection of improved varieties of Korla fragrant pear. Background technique [0002] Korla fragrant pear is a famous, excellent and characteristic fruit in Xinjiang. It has been cultivated for more than 1,400 years. It is produced on the banks of the Peacock River in Bazhou, southeastern Xinjiang. It is mainly distribu...

AI Technical Summary

Problems solved by technology

[0003] Aiming at the defect that the safranin-fast green dyeing method cannot clearly color all the cells in the Korla fragrant pear fruit, the problem to be solved in the present invention is to provide a three-dimensional dyeing method that is easy to operate, and can perform three-dimensional staining on the Korla fragrant pear fruit cells , cortical cells, pulp cells, stone cells, and conducting tissue cells are clearly stained and clearly differentiated from each other