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A three-dimensional cell capture and release chip and its preparation method

A three-dimensional structure and cell technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc. and other problems, to achieve the effect of high efficiency, short capture time and simple processing technology

Active Publication Date: 2017-05-31
WUHAN LANDING INTELLIGENCE MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the channels of microfluidic chips etched by traditional chemical etching methods are very regular, and microfluidic chips with special-shaped microfluidic channels cannot be etched.
Traditional etching methods cannot etch microfluidic chips with special-shaped channels

Method used

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  • A three-dimensional cell capture and release chip and its preparation method
  • A three-dimensional cell capture and release chip and its preparation method
  • A three-dimensional cell capture and release chip and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Such as Figure 1-2 As shown, a three-dimensional cell capture and release chip includes an upper cover 1 and a lower substrate 2, the back of the upper cover 1 is etched with a microfluidic channel 4, and the microfluidic channel 4 of the cover 1 is two An inlet 3 and an outlet 4 are respectively arranged at the ends, a layer of chitosan film 6 is provided on the substrate 2, and the cover sheet 1 and the substrate 2 are bonded by an adhesive.

[0038] The preparation method of the cell capture and release chip with the above three-dimensional structure, the steps are as follows:

[0039] (a) Select glass, silicon wafer or quartz as the material of the cover and substrate, and clean the selected cover and substrate to remove impurities such as oil on the surface;

[0040] (b) Dry the cleaned cover and substrate in an oven at 50°C to 100°C. After drying, adhere a chemical-resistant mask to the upper and lower surfaces of the cover, and cut it by laser. Cut out part of...

Embodiment 2

[0047] A method for preparing a three-dimensional cell capture and release chip, the steps are as follows:

[0048] (a) Select the material or glass of the cover and substrate as glass, silicon wafer or quartz, and clean the selected cover and substrate to remove impurities such as oil stains on the surface;

[0049] (b) Dry the cleaned cover and substrate in an oven at 50°C to 100°C. After drying, adhere a chemical-resistant mask to the upper and lower surfaces of the cover, and cut it by laser. Cut out part of the area to be etched, remove the anti-corrosion mask cut out, and obtain the area to be etched;

[0050] (c) Put the processed cover sheet into the etching solution vertically and slowly at the rate of 0.01mm / min or 0.1mm / min or 0.25mm / min or 0.75mm / min or 1mm / min through the fixture for etching Etching, etch for 1 hour, because there is a sequence of entering the etching solution, so the etching time is different, resulting in different etching depths, so the microflu...

Embodiment 3

[0056] A method for capturing and releasing cells based on a chip with a three-dimensional structure, the steps of which are as follows:

[0057] (1) if Figure 3-4 As shown, the capture of cells:

[0058] (a) inject the liquid sample containing ctc circulating tumor cells to be detected into the chip of Example 1 through the syringe pump at a rate of 0.51ul / min from the inlet hole;

[0059] (b) Continue to inject phosphate buffer solution (i.e. PBS solution) at the inlet, and inject at a speed of 50ul / min for cleaning;

[0060] (c) Inject tracer substances (such as: fluorescent dyes such as DAPI or Hoechst dyes that stain the nucleus) at the entrance to identify target cells;

[0061] (d) Inject phosphate buffer solution at the inlet again for cleaning, and place the chip under a microscope to observe the captured target cells;

[0062] (2) if Figure 5-6 As shown, the cells release:

[0063] (a) The chip captures cells with a specific capture size stuck in the correspon...

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Abstract

The invention relates to a three-dimensional structural cell capture and release chip, which comprises an upper cover plate and a lower substrate. A microflow channel is etched on the back of the upper cover plate. Two ends of the microflow channel of the cover plate are respectively provided with an inlet and an outlet. A layer of chitosan film is arranged on the substrate. The cover plate and the substrate are glued through an adhesive. By the utilization of the structure, cells / particles can be captured and released. A liquid to be measured passes through the three-dimensional structural channel of the micro-fluidic chip to capture cells / particles with specific size by height characteristics of the three-dimensional structure. After capture, height of the internal channel of the three-dimensional chip is increased by a specific method so as to finally release cells / particles. The operational method of cell capture and release is convenient, has fast response, doesn't damage cells and is suitable for capturing and releasing micro-scale cells and particles. The preparation method has a simple process. The prepared three-dimensional structural chip has characteristic of miniaturization and can be widely used in the field of analysis.

Description

technical field [0001] The invention relates to a preparation method of a microfluidic chip, in particular to a three-dimensional structure cell capture and release chip, and a method for capturing and releasing cells by using the chip, which is suitable for the capture and release of micron-scale cells and particles. Background technique [0002] The microfluidic chip is the core of the microanalysis system. The invention and application of the microfluidic chip has provided great convenience to the scientific fields such as chemistry, food, environment, and medicine, and promoted the miniaturization, integration, automation and convenience of some analyzes . It greatly saves the consumption of some chemical reactions on expensive chemical reagents, and also greatly improves the analysis efficiency and reduces the cost. [0003] Usually, microfluidic chips that capture cells mainly use mechanical separation methods, electrical induction separation methods, and magnetic man...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
Inventor 张南刚周琼伟刘侃刘松林
Owner WUHAN LANDING INTELLIGENCE MEDICAL CO LTD
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