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Regulation and control method for fermentation process of L-arginine high in acid yield

A fermentation process and arginine technology, which is applied in the field of regulation of high-acid-yielding L-arginine fermentation process, and can solve problems such as the reduction of conversion rate and production intensity.

Inactive Publication Date: 2015-08-05
NINGXIA WANSHENG BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And when trying to obtain higher product concentration with batch fermentation, it will inevitably mean further decline in conversion rate and production intensity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Use Brevibacterium flavum. In the 300L seed culture medium (PH6.7-7.0) that is made up of glucose 4.0%, corn steep liquor 1.5%, ammonium sulfate 0.3%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, control tank pressure 0.05-0.06MPa, The rotation speed is 200-250rpm, the culture temperature is 30-32°C, and after 18 hours of culture, add 15% glucose, 2% corn steep liquor, 1% molasses, 0.3% ammonium sulfate, 0.05% potassium dihydrogen phosphate, and 0.05% magnesium sulfate 2200L In the fermentation broth, the stirring speed is 220rpm, and the cultivation temperature is 30-32°C. The pH of the whole fermentation process is controlled at 6.8-6.9, and the dissolved oxygen is controlled at 20-30%.

[0025] The early stage is mainly the period of long bacteria, and the sugar consumption is relatively slow. When the bacteria grow to a certain stage and begin to transform, at this time, the microscopic examination can clearly find that the number of "eight" split...

Embodiment 2

[0030] Use Brevibacterium flavum. In the 300L seed culture medium (PH6.7-7.0) that is made up of glucose 4.0%, corn steep liquor 1.5%, ammonium sulfate 0.3%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, control tank pressure 0.05-0.06MPa, The rotation speed is 200-250rpm, the culture temperature is 30-32°C, and after 18 hours of culture, add 15% glucose, 2% corn steep liquor, 1% molasses, 0.3% ammonium sulfate, 0.05% potassium dihydrogen phosphate, and 0.05% magnesium sulfate 2200L In the fermentation broth, the stirring speed is 220rpm, the culture temperature is 30°C, and the process is controlled. When the OD is below 0.8, the pH is controlled at 6.8-6.9, and the dissolved oxygen is 20-30%. After the OD0.8 is above, the pH is reduced to 6.5-6.6. Control at 10-20%, sugar concentration drops below 1%, start to add sugar, control sugar concentration at 0.5-1%. During the whole process, the growth rate of bacteria in the early stage was significantly accelera...

Embodiment 3

[0034]Use Brevibacterium flavum. In the 300L seed culture medium (PH6.7-7.0) that is made up of glucose 4.0%, corn steep liquor 1.5%, ammonium sulfate 0.3%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%, control tank pressure 0.05-0.06MPa, The rotation speed is 200-250rpm, the culture temperature is 30-32°C, and after 18 hours of culture, add 13% glucose, 2% corn steep liquor, 1% molasses, 0.3% ammonium sulfate, 0.05% potassium dihydrogen phosphate, and 0.05% magnesium sulfate 2200L In the fermentation broth, the stirring speed is 220rpm, the culture temperature is 30-32°C, and the process is controlled. When the OD is below 0.8, the pH is controlled at 6.8-6.9, and the dissolved oxygen is 20-30%. After the OD is above 0.8, the pH is reduced to 6.5-6.6. Dissolved oxygen is controlled at 10-20%, the sugar concentration drops below 2%, and sugar is added continuously, and the sugar concentration is controlled at 1-2%. During the whole process, the growth rate of...

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Abstract

The invention relates to a regulation and control method for a fermentation process of L-arginine high in acid yield. According to the method, when brevibacterium flavum is used as a seed for fermentation production of the L-arginine, temperature, stirring speed and tank pressure of the fermentation process and pH values and dissolved oxygen quantity of different fermentation stages are controlled online by an FPC (flexible printed circuit) type distributed control system, bacterium cell concentration, substrate concentration and product yield during fermentation are measured through timing sampling, and carbohydrates are fed and supplemented when sugar concentration is lower than a critical value. The regulation and control method has the advantages that the distributed control system is adopted for biochemical processes, so that the method is simple and reliable and high performance is achieved; through optimization control of the fermentation process, the acid yield of the L-arginine is increased by 15-20%, fermentation period is shortened to 76-86h, the volume of a lower tank of a fermentation tank is increased from 70% to 80% and stability is high.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a method for regulating and controlling the fermentation process of L-arginine with high acid yield. Background technique [0002] In the 1940s, when nutritionists divided amino acids into essential amino acids and non-essential amino acids, they believed that L-arginine was a non-essential amino acid for adults and animals, so it was not included in the essential amino acids. It was later found to be insufficiently synthesized by itself in infants and young animals and is now called a semi-essential amino acid. In the 1980s, through the extensive application and in-depth research of amino acid additives in the feeding industry, it was found that L-arginine is an essential amino acid for aquatic animals such as chickens and fish. [0003] At present, the production methods of L-arginine mainly include hydrolysis and fermentation. [0004] Pig hair, hoof nails, b...

Claims

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Application Information

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IPC IPC(8): C12Q3/00C12P13/10C12R1/13
Inventor 王唐高建涛陈桃生廖飞徐振华仇俊鹏
Owner NINGXIA WANSHENG BIO ENG
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