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Formulas of rapid propagation culture media of oncidium

A culture medium formula and proliferation medium technology, applied in the field of oncidium rapid propagation medium formula, can solve the problems of virus affecting plant growth and flower appreciation, unsatisfactory culture effect, high transplanting mortality, etc., to improve the resistance The effect of improving the resistance and transplanting survival rate of tissue culture seedlings, improving the environmental adaptability of seedlings

Active Publication Date: 2014-09-10
INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Many oncidium varieties have been introduced into my country in recent years, but the production of seedlings mainly depends on ramets, which not only reproduces slowly, but also accumulates viruses that seriously affect the growth of plants and the ornamental quality of flowers.
The use of plant tissue culture technology can solve the problem of rapid propagation of oncidium high-quality seedlings. At present, there are reports and patents on oncidium tissue culture in China, but the culture effect is not ideal. The resistance of tissue culture seedlings is poor, and the transplanting mortality rate is high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Propagation object: protocorms of oncidium explants

[0026] The cultivation process is divided into three stages, as follows:

[0027] The first stage: Cultivate protocorm proliferation medium, inoculate oncidium protocorm into the protocorm proliferation medium for culture, the medium formula is: MS+6-BA 3.0mg / L+NAA 0.25mg / L+coconut juice 100ml / L + sucrose 30g / L + agar 5.5g / L, pH 5.5; Proliferation culture 15d-25d, protocorm proliferation culture ends.

[0028] The second stage: strong seedling cultivation, the protocorm is connected to the strong seedling medium for cultivation, the medium formula is: MS+peptone 2.0g / L+banana 30g / L+potato 30g / L+sucrose 25g / L+agar 5.5g / L, PH 6.0; Strong seedlings are cultivated for 25d-35d, and the strong seedlings are cultivated.

[0029] The third stage: rooting culture, inoculate the single-plant tissue culture seedlings with a height of more than 1cm obtained from strong seedling cultivation into the rooting medium for cultivat...

Embodiment 2

[0033] Propagation object: protocorms of oncidium explants

[0034] The cultivation process is divided into three stages, as follows:

[0035] The first stage: protocorm proliferation medium, oncidium protocorm is inoculated into the protocorm proliferation medium for culture, the medium formula is: MS+6-BA 3.5mg / L+NAA 0.3mg / L+coconut milk 150ml / L + sucrose 35g / L + agar 5.5g / L, PH 5.8; Proliferation culture 15d-25d, protocorm proliferation culture stage ends;

[0036] The second stage: strong seedling cultivation, the protocorm is connected to the strong seedling medium for cultivation, the medium formula is: MS+peptone 2.5g / L+banana 35g / L+potato 35g / L+sucrose 30g / L+agar 5.5g / L, PH 6.0; The strong seedlings are cultivated for 30d-35d, and the single tissue cultured seedlings with a size of 1cm or more are obtained, and the strong seedlings are cultivated.

[0037] The third stage: rooting culture, inoculate the single plant tissue culture seedlings with a height of more tha...

Embodiment 3

[0041] Propagation object: protocorms of oncidium explants

[0042] The cultivation process is divided into three stages, as follows:

[0043]The first stage: protocorm proliferation medium, oncidium protocorm is inoculated into the protocorm proliferation medium for cultivation, the medium formula is: MS+6-BA 2.0mg / L+NAA 0.2mg / L+coconut juice 80ml / L + sucrose 25g / L + agar 5.5g / L, pH 5.5; Proliferation culture 18d-30d.

[0044] The second stage: strong seedling cultivation, after 18d-30d of proliferation culture, connect the protocorm to the strong seedling medium for cultivation, the medium formula is: MS+peptone 1.5g / L+banana 25g / L+potato 25g / L+sucrose 20g / L+ Agar 5.5g / L, pH 6.0; Strong seedlings were cultured for 30d-40d to obtain single tissue cultured seedlings with a height of more than 1cm.

[0045] The third stage: rooting culture, inoculate the single-plant tissue culture seedlings obtained from the cultivation of strong seedlings with a height of more than 1cm int...

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Abstract

The invention discloses formulas of rapid propagation culture media of oncidium. The rapid propagation culture media comprise a protocorm multiplication culture medium, a strong seedling culture medium and a rooting culture medium in three stages. According to the formulas, after about 3-4 months, oncidium protocorm of explants can undergo the three stages of protocorm multiplication culture, strong seedling culture and rooting culture so as to become rooted oncidium with relatively high transplanting survival rate, so that the rapid propagation capacity of high-quality seedlings of oncidium is improved, the environment adaptive capacity of the oncidium seedlings is enhanced, the resistance and the transplanting survival rate of the tissue culture seedlings of oncidium are improved, the transplanting survival rate is above 97% and then oncidium is generally popularized and applied in China.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a formula of oncidium rapid propagation medium. Background technique [0002] Oncidium, also known as Dancing Girl Orchid, Dancing Orchid, Tumor Petal Orchid, belongs to the genus Oncidium of Orchidaceae. There are more than 400 native species in the genus. It is native to Mexico, West Indies, Brazil and other places. It has a wide distribution area, many varieties, and great differences in growth habits. It is one of the large genera of orchids. The variety is mostly golden yellow with brown spots. In the research, pink, purple, pure yellow, wine red and other excellent varieties with beautiful flowers and perennial flowering were bred through interspecific and intergeneric hybridization. Oncidium's special shape, straight and long pedicels, rich colors, and long flowering period, so as an important cut flower and potted flower species, the market demand has incre...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张本厚胡燕花陈集双
Owner INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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