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Male trait induction method for pelodiscus sinensis in embryonic periods

An embryonic soft-shelled soft-shelled turtle technology, applied in diseases, medical formulas, organic active ingredients, etc., can solve problems such as abnormal development of gonads in embryonic development, difficulty in achieving sex reversal, inability to achieve masculinization of individuals, etc., and achieve a large hatching capacity , easy to operate, beneficial to the effect of embryonic development in ovo

Active Publication Date: 2014-04-23
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technique has the following two defects: ①Although methyltestosterone can induce the external sexual characteristics of soft-shelled turtle, because the aromatase existing in the female body can convert testosterone into estrogen, when treated with methyltestosterone alone It is difficult to achieve a complete sex reversal effect, and it is impossible to masculinize all individuals
② This method needs to add medicine dropwise every day, and the operation is cumbersome, and high doses of methyl testosterone can easily lead to abnormal development of embryos and gonads in the future

Method used

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  • Male trait induction method for pelodiscus sinensis in embryonic periods
  • Male trait induction method for pelodiscus sinensis in embryonic periods
  • Male trait induction method for pelodiscus sinensis in embryonic periods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. Preparation of inducer:

[0055] Dissolve aromatase inhibitors and auxiliary agents in alcohol, and mix well to obtain different concentrations of inducers. The specific situation of each inducer is shown in Table 1.

[0056] Table 1

[0057]

[0058] Use above-mentioned incubator to carry out the induction method of the male sex of soft-shelled turtle embryo stage as follows:

[0059] Collect the fertilized eggs within 12 hours after laying eggs of soft-shelled turtles, put the soft-shelled turtle eggs directly in the positioning hole of the incubation plate, put them into the box, set the incubation temperature at 31°C, turn on the power, and the control module controls the heating device, ultrasonic The humidifier and fan worked. The humidity is controlled at 75% to 85%. After 1 day of hatching, the unfertilized embryos were removed, and the fertilized soft-shelled turtle eggs continued to hatch.

[0060] 2. Drug treatment:

[0061] Select seven incubator...

Embodiment 2

[0072] 1. Inducer configuration

[0073] Dissolve aromatase inhibitors and auxiliary agents in alcohol, and mix well to obtain different concentrations of inducers. The specific situation of each inducer is shown in Table 3.

[0074] table 3

[0075]

[0076] The incubation method and the application method of the inducer are the same as in Example 1.

[0077] 2. Drug treatment:

[0078] Using the above-mentioned A1 to F1 inducers to induce the turtle eggs at the embryonic stage and the hatching methods in the middle and late stages are all the same as those in Example 1.

[0079] The juvenile soft-shelled soft-shelled turtles were collected, the hatching rate and average body weight were counted, and then the gonads were slaughtered for histological sex identification. The results of hatching rate and gender identification are shown in Table 4.

[0080] Table 4

[0081]

[0082] It can be seen from Table 4 that the hatchability and average body weight of soft-shel...

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Abstract

The invention relates to a male trait induction method for pelodiscus sinensis in embryonic periods. The male trait inducer is characterized in that through dissolving an aromatase inhibitor and an adjuvant into alcohol with the purity of 92-98%, the inducer with an aromatase inhibitor concentration of 50-150 mu g / mu l and an adjuvant concentration of 50-150 mu g / mu l is obtained; and the adjuvant is selected from testosterone, methyltestosterone or testosterone propionate. According to the induction method provided by the invention, the male induction rate can be more than 95% just through spraying the inducer two times at the sex determination key time point in embryonic development, and meanwhile, the normal development and low drug residues of pelodiscus sinensis gonads are ensured; and the induction method provided by the invention is easy to operate, low in cost, and remarkable in economic benefit, thereby being a sex control technology which is worthy of popularization in the existing pelodiscus sinensis breeding.

Description

technical field [0001] The invention relates to the field of aquatic product cultivation, in particular to a method for inducing male sex in the embryonic stage of soft-shelled turtle. Background technique [0002] Turtle is a traditional high-grade nourishing aquatic product in my country, and it is also a precious aquatic product and an important species of aquaculture loved by the people. It plays a pivotal role in my country's aquaculture industry. In the long-term breeding practice of soft-shelled turtles, it has been found that there are great growth differences between male and female individuals. Male soft-shelled turtles grow significantly faster than female soft-shelled turtles. According to experiments, male soft-shelled turtles grow 12.7% to 17.0% faster than female soft-shelled turtles in groups with an average weight of 300g. With the extension of the breeding period, the growth of female and male soft-shelled turtles The difference will further increase. Male...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K31/568A01K67/02A61P15/00
Inventor 钱国英葛楚天王莉汪财生王伟李彩燕
Owner ZHEJIANG WANLI UNIV
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