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Single Nucleotide Polymorphism Site of Goat sh2b1 Gene and Its Detection Method

A single nucleotide polymorphism, SH2B1 technology, applied in the field of molecular genetics, to achieve the effect of low cost and high accuracy

Inactive Publication Date: 2017-10-24
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

The domestic and foreign studies on the genetic variation of the SH2B1 gene in livestock, except for the recent SNP of the SH2B1 gene and the growth traits of Chinese cattle, have not yet been reported in goats

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  • Single Nucleotide Polymorphism Site of Goat sh2b1 Gene and Its Detection Method
  • Single Nucleotide Polymorphism Site of Goat sh2b1 Gene and Its Detection Method
  • Single Nucleotide Polymorphism Site of Goat sh2b1 Gene and Its Detection Method

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Embodiment Construction

[0031] In order to facilitate the understanding of the technical solutions of the present invention, the present invention will be further described below with reference to specific embodiments.

[0032] The present invention designs primers according to the conserved sequence of the SH2B1 gene, respectively uses the genomic DNAs of four goat populations as templates, carries out PCR amplification, mixes the PCR products, purifies them, and sequences them. Then, sequence map analysis and sequence alignment were performed to screen out SNP sites; secondly, PCR-SSCP detection of polymorphic sites was performed in the population to be tested; finally, population genetic statistical analysis was performed according to the genotypes detected in the population Association analysis with body size traits, and screened out molecular markers closely related to goat body size traits. The present invention will be described in detail below, which is to explain rather than limit the presen...

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Abstract

The invention discloses goat SH2B1 gene single nucleotide polymorphism (SNP) loci and a detection method of the loci. The goat SH2B1 gene is subjected to PCR (polymerase chain reaction) amplification with the goat full-genome DNA to be detected comprising the SH2B1 gene as a template and a primer pair of P1 and P2 as primers, and SSCP (single-strand conformation polymorphism) detection is carried out. The gene polymorphism loci comprise the polymorphism that the 4356th site of the goat SH2B1 gene is T or C base, and the polymorphism that the 6033th site of the goat SH2B1 gene is G or A base. According to the goat SH2B1 gene single nucleotide polymorphism (SNP) loci and a detection method of the loci provided by the invention, PCR product mixed sequencing for screening SNP and PCR-SSCP are combined, so that the SSCP instability is solved and the missed detection of the mutant site is eliminated; and the detection method is simple, rapid, low in cost, high in precision, and convenient for popularization and application, can be used for screening and detecting genetic markers closely related to goat growth traits on a DNA (deoxyribonucleic acid) level, and can be used for assisted selection and molecule breeding of the goat.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a goat SH2B1 gene single nucleotide polymorphism site and a detection method thereof. Background technique [0002] Single nucleotide polymorphism (SNP) refers to the polymorphism caused by the substitution of a single nucleotide (A / T / C / G) in the genomic DNA sequence, mainly caused by the conversion or transversion of a single base . About two-thirds of the SNPs had transformational variants, and several other SNPs were at similar levels. The cytosine of CpG dinucleotides is the most mutated site in the genome, most of which are methylated and can spontaneously deaminate to form thymine. [0003] According to the influence on genetic traits, gene polymorphism can be divided into two types: one is synonymous mutation polymorphism, that is, the change of the coding sequence caused by SNP does not affect t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 陈宏刘宣宣房兴堂张春雷
Owner XUZHOU NORMAL UNIVERSITY
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