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Determination method for total flavone content in pollen pini supercritical extractant

A technology of supercritical extract and determination method, which is applied in the field of determination of total flavonoid content in supercritical extract of pine pollen, and achieves the effect of good precision

Inactive Publication Date: 2012-04-25
JIANGSU UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, there are no literatures and patents on the content determination of flavonoids in the supercritical extract of pine pollen at home and abroad.

Method used

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  • Determination method for total flavone content in pollen pini supercritical extractant
  • Determination method for total flavone content in pollen pini supercritical extractant
  • Determination method for total flavone content in pollen pini supercritical extractant

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Embodiment Construction

[0018] The present invention will be further described below by specific examples.

[0019] 1. Experimental part

[0020] 1. Instruments and reagents

[0021] UV-2401PC ultraviolet spectrophotometer (Shimadzu, Japan); XT-102A electronic balance (Switzerland Precisa), etc.

[0022] Pine Pollen Supercritical CO 2 Extract (SFE-PP, Jiangsu Jiangdayuan Ecological Biotechnology Co., Ltd.); Rutin reference substance (Nanjing Zelang Pharmaceutical Technology Co., Ltd.); other reagents were of analytical grade (Sinopharm Chemical Reagent Co., Ltd.).

[0023] 2. Experimental method Determination of total flavonoids in SFE-PP:

[0024] 2.1 Preparation of standard stock solution: Accurately weigh an appropriate amount of rutin reference substance, add methanol to dissolve and dilute to 100ml, and make 150μg·ml -1 standard stock solution of rutin.

[0025] 2.2 Preparation of test sample solution:

[0026] 2.2.1 Accurately weigh 2.0000g of pine pollen supercritical extract, wrap it ti...

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Abstract

The invention relates to a determination method for a total flavone content in a pine pollen supercritical extractant. According to the method, rutin is adopted as a standard material; 70% ethanol is adopted to extract the pollen pini supercritical extractant; a polyamide resin chromatographic column is adopted to separate the total flavone; a 5% sodium nitrite solution, a 10% aluminum nitrate solution and a sodium hydroxide solution with the concentration of 1.0 mol.L<-1> are added to carry out a color reaction; the absorbance is detected at the ultraviolet light wave length of 510 nm to detect the total flavone content. According to the present invention, a simple and feasible analysis scheme is established for the total flavone content in the pollen pini extracted by supercritical fluid extraction, the accuracy and the precision are good, and a method basis is provided for quality control of the pollen pini supercritical extractant.

Description

technical field [0001] The invention relates to a method for determining the content of total flavonoids in a supercritical extract of pine pollen. Background technique [0002] Pine pollen (Pollen Pini) is obtained from plants such as Pinus massonina lamb (Pinus massonina lamb) and Chinese pine (Pinus tabulaefermiscarr) in spring when the male cones are just opened, dried in the sun, rubbed pollen, collected, and removed from impurities. Yellow fine pollen [see: ChP (Chinese Pharmacopoeia). 2010. Vol I (part one): 191]. Pine pollen is nature's micro-nutrient reservoir. As an excellent health food, the nutritional components of pine pollen can be divided into six categories: sugar, protein, fat, vitamins, trace elements and biologically active substances. It contains a variety of enzymes, hormones, flavonoids and rich phospholipids and other biologically active substances that have an important role in regulating the life activities of the human body. A variety of pharmac...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/40
Inventor 余江南童姗姗徐希明邓倩
Owner JIANGSU UNIV
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