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Antibiotic titer determination method based on ink-jet printing principle

An inkjet printing and potency measurement technology, applied in printing, testing pharmaceutical preparations, material inspection products, etc., can solve problems such as droplet rupture, test failure, and cumbersome operation, and achieve error reduction, accuracy and repeatability. Good, simple and fast effect

Inactive Publication Date: 2013-11-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method needs to carefully place a small steel pipe on the surface of the culture medium coated with bacteria, and manually add the antibiotic solution into the small steel pipe, and requires a large number of repeated experiments in multiple batches. Not only is the operation cumbersome, It is time-consuming and labor-intensive, and more importantly, human factors in the test will inevitably cause deviations between test batches. Different personnel operations may obtain inconsistent test results, and the measurement error is large. Therefore, the reliability and reproducibility of the test sex is also relatively poor
The impact of some unfavorable human factors in the tube-and-disk method of antibiotic potency determination is specifically reflected in: (1) Due to the instability of manual operation, the amount of antibiotic solution added to small steel pipes of the same size may be different, and the amount of antibiotic solution may be different. The concentration of diffusion will be different, and the size of the final formation of the inhibition zone will also change, and the difference between the deviation from parallel and the plate will increase, which will affect the accuracy of the test; (2) when placing the small steel pipe by hand, there may be uneven force Defects, which lead to uneven diffusion of antibiotics in the agar and affect the roundness of the inhibition zone
(3) In the process of dropping antibiotics with a dropper or pipette, it is easy to cause the droplet to break and splash out of the small steel pipe, which will lead to the failure of the test

Method used

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  • Antibiotic titer determination method based on ink-jet printing principle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Select a Canon PIXMA ip1880 thermal jet color inkjet printer with a resolution of 1200×4800d.p.i. as a tool for dropping antibiotics, firstly modify the main body of the printer: remove the shell of the main body of the printer, and remove the roll located under the ink cartridge Paper device and waste ink removal device, and the plastic base below it is sawed off, and the agar glass double dish coated with bacteria layer can be placed in the vacant position. Afterwards, modify the ink cartridge: pry off the outer cover, use tweezers to clamp out the sponge full of ink, and rinse the ink at the bottom of the ink cartridge with clean water until there is no residue. Finally, take out the filter screen located above the nozzle, and rinse it repeatedly with clean water. Before loading antibiotics, wash it with 70% ethanol for 3-5 times. After air-drying, place it under a UV lamp to sterilize for about 1 hour;

[0025] 2) In this example, according to the two-dose method...

Embodiment 2

[0036] 1) step 1 is the same as step 1 in embodiment 1;

[0037] 2) step 2 is the same as step 2 in embodiment 1;

[0038] 3) Except that the experimental bacteria used were changed to Bacillus pumilus, all the other steps were the same as step 3 in Example 1;

[0039] 4) Preparation and printing of standard gentamicin and gentamicin to be tested: use phosphate buffer (0.2% dipotassium hydrogen phosphate, 0.8% potassium dihydrogen phosphate, pH6.0, sterilize at 115°C for 30min) Fully dissolve the standard gentamicin and the gentamicin powder to be tested respectively, prepare the standard gentamicin and the gentamicin to be tested at a concentration of 4 mg / ml, and take out 100 μl of the prepared gentamicin standard Add the sample to the black ink cartridge, take out 100 μl of prepared gentamicin to be tested and drop it into the magenta ink cartridge as the "ink" for printing; place the agar glass double dish containing Bacillus pumilus in step 3) on the 1mm below the nozzl...

Embodiment 3

[0048] 1) step 1 is the same as step 1 in embodiment 1;

[0049] 2) step 2 is the same as step 2 in embodiment 1;

[0050] 3) Except that the experimental bacteria used were changed to Staphylococcus aureus, all the other steps were the same as step 3 in Example 1;

[0051] 4) Preparation and printing of standard streptomycin and streptomycin to be tested: use phosphate buffer (dipotassium hydrogen phosphate 0.2%, potassium dihydrogen phosphate 0.8%, pH 6.0, sterilize at 115°C for 30 minutes) Dissolve the streptomycin standard substance and the streptomycin powder to be tested, prepare the streptomycin standard substance and the streptomycin to be tested at a concentration of 5 mg / ml, take out 100 μl of the prepared streptomycin standard substance and add it dropwise to the black ink cartridge, take out Add 100 μl of the prepared streptomycin to be tested dropwise into the magenta ink cartridge as the "ink" for printing; place the agar glass double dish containing Staphylococ...

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PUM

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Abstract

The invention discloses an antibiotic titer determination method based on an ink-jet printing principle. A reconstructed commercialized thermal inject color printer is used as an antibiotic distribution tool to replace a manual dropper or pipette gun for antibiotic distribution. Besides, the invention employs a specific patterning edited by software to replace a small steel pipe in a plate assay, and precisely controls factors of a printed antibiotic type, a printing amount and a printing position, so as to realize a same antibiotic titer determination as the plate assay based on a diffusing bacteriostasis principle. According to the method, the small steel pipe does not need to be overlaid on the agar glass double discs, and a tedious manual dropping process is omitted to reduce errors in manual operation, so as to realize simpleness, rapidness, high precision and good reappearance.

Description

technical field [0001] The invention relates to a method for measuring the potency of antibiotics, in particular to a method for measuring the potency of antibiotics based on the principle of inkjet printing, and belongs to the technical field of drug detection. Background technique [0002] Antibiotic potency is an index to measure the quality or biological efficacy of antibiotics. Accurate determination of antibiotic potency has important guiding significance in the fields of medicine, immunization, and clinical diagnosis. The most commonly used method for the determination of antibiotic potency is the tube-and-dish method. The tube-and-disk method of antibiotic titer measurement is based on the antibacterial efficacy of antibiotics against microorganisms as the measure of potency. It has the advantages of being consistent with the application principle, less dosage and high sensitivity. method. [0003] The principle of antibiotic potency determination is that when anti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/15B41J2/21
Inventor 徐志南郑强黄磊蔡谨
Owner ZHEJIANG UNIV
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