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Simple method for quickly collecting large quantity of female root-knot nematodes

A technology for root-knot nematodes and female insects, applied in the preparation of test samples, etc., can solve the problems of time-consuming and labor-intensive insects, easy to break, etc., and achieve the effect of simple method, good integrity, and large number of female insects

Inactive Publication Date: 2013-08-28
吴海燕
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Problems solved by technology

[0003] The purpose of the present invention is to provide a simple method that can obtain a large number of complete female root-knot nematodes in a short period of time, so as to overcome the time-consuming and labor-intensive shortcomings of the existing technology for obtaining female root-knot nematodes and the fragility of the insect body

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  • Simple method for quickly collecting large quantity of female root-knot nematodes
  • Simple method for quickly collecting large quantity of female root-knot nematodes

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Embodiment Construction

[0022] The technical solutions of the present invention will be described in detail below in conjunction with specific embodiments.

[0023] 1. Prepare disease roots with obvious root-knot symptoms, 500ml beaker, tissue grinder, 20-mesh and 80-mesh standard sieves, stereo dissecting mirror, constant temperature incubator.

[0024] 2. Cut the diseased root into 2cm lengths and put it in a 500ml beaker, add tap water to soak the root tissue, and place it in a 30°C incubator overnight. When the water in the beaker is cloudy, take out the root and move it into a tissue grinder, and work for 30 seconds. Then by 20 mesh and 80 mesh cover sieves, collect the residue on the 80 mesh sieves, observe a large amount of white females under the dissecting microscope, as figure 1 Middle A (bar=1000 μm) picture and B (bar=500 μm) picture.

[0025] 3. Pick the white female worms of root-knot nematode directly under a stereo dissecting microscope or in a well-lit place with a fine brush.

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Abstract

The invention discloses a simple method for quickly collecting a large quantity of female root-knot nematodes. The method comprises the following steps of: decomposing epithelial tissues and cortical tissues of the roots of decayed plants by using bacteria at the temperature of 25-30 DEG C; removing epidermis and cortexes of the roots of decomposed decayed plants, releasing internal females, sieving with a 20-mesh sieve and a 80-mesh sieve and collecting residues on the 80-mesh sieve; and directly picking white female root-knot nematodes with a thin writing brush under a stereoscopic anatomical lens or at a light-sufficient place. By adopting the method, a large quantity of female root-knot nematodes can be collected within a short period of time, no special instrument equipment is needed, and the defects such as time wasting, labor wasting and easily breaking of nematode bodies in the conventional technology are overcome.

Description

technical field [0001] The invention relates to a simple method for obtaining a large amount of female root-knot nematodes. It is suitable for the research of phytopathology and phytonematology, the experimental teaching courses of phytopathology and phytonematology, and has extremely important application value in the taxonomy of root-knot nematodes. Background technique [0002] The perineal pattern is an important morphological basis for the identification of root-knot nematode species. A large number of females are needed to prepare the slides of the perineal pattern and experimental teaching, and the female root-knot nematodes are all buried in the root tissue of the host. To obtain the females must be The root knot is dissected under a dissecting microscope, and the internal females are taken out. During the operation, the internal females cannot be seen clearly. Therefore, the females are often broken during dissection, so that it takes a lot of time to obtain a small...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
Inventor 吴海燕周勋波毕建杰
Owner 吴海燕
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