Method for introducing radish chromosomes into cabbage

Abstract

The invention discloses a method for introducing radish chromosomes d into cabbage. The method comprises the following steps of: performing sexual hybridization on a cabbage type rape-radish chromosome d addition line and cabbage; introducing the radish chromosomes d into the cabbage; selecting a cabbage plant containing the radish chromosomes d by adopting a molecular marker detection and cell microscopy method; and creating a new cabbage germplasm carrying the radish chromosomes d. In the method, cabbage type rape is taken as a carrier of the radish chromosome d, and is hybridized with cabbage to transfer the radish chromosome d to the cabbage, so that direct hybridization between the cabbage and radish is avoided. Hybridization in brassica alboglabra succeeds easier than intergeneric hybridization between brassica alboglabra and radish. Moreover, only one pair of radish chromosomes is integrated with a cabbage genome, so that more genetic drag caused by the hybridization of an entire radish genome can be avoided, and the transfer and selection efficiencies of target traits can be improved. By adopting the method, a novel thought is provided for distant hybridization utilization.

Description

technical field [0001] The invention relates to a cultivation method of cabbage, in particular to a method for introducing d-chromosome of radish into cabbage, and belongs to the field of distant hybridization and utilization of cabbage. Background technique [0002] Distant hybridization refers to the hybridization between distantly related types, including hybridization between species, genera and families. It can create specific types or new germplasm that existing plants do not have, so distant hybridization has become an important means to solve the shortage of plant genetic resources, and has been widely used in plant genetics and breeding, such as in wheat (Bris B. et al., 1983; Kozub NA et al., 2004), cotton (Vmh B I et al., 1999), rape (Liu Zhongsong et al., 2001; Chen Shuzhong et al., 2000), cucumber (Chen J F et al., 1997) and other crops through interspecific or intergenus hybridization Variety improvement has been carried out to obtain rich genetic variation ty...

AI Technical Summary

Problems solved by technology

Compared with sexual hybridization, although protoplast fusion technology can overcome the incompatibility of distant hybridization to a certain extent and obtain hybrids, it is still difficult to achieve in terms of the technical maturity of protoplast culture, the genetic stability and fertility of somatic hybrids. (especially in distant somatic cell hybridization) and the randomness of chromosome disappearance in asymmetric hybridization makes it impossible to transfer specific traits in a targeted manner. There are problems to be studied and solved urgently (Song Shangwei et al., 2007)

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