Method for inhibiting movement of flounder eyes
A flounder and eye technology, which is applied in the field of inhibiting flounder eye movement, can solve problems such as small natural mutation, and achieve the effect of obvious effect and single effect.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0050] The method of microinjecting colchicine to inhibit the movement of the right eye of flounder can be operated according to the following steps:
[0051] 1. Preparation of colchicine
[0052] Dilute the colchicine into a 5 mg / ml stock solution of colchicine with sterile 0.75% sodium chloride solution to prepare a 50 μg / ml colchicine solution.
[0053] 2. Preparation of anesthetics
[0054]Use freshly aerated seawater with a salinity of 35‰ and a temperature of 20°C to prepare a 0.1mg / mL solution of ethyl 3-aminobenzoate methanesulfonate (tricaine methanesulfonate, MS-222), and prepare it immediately before use.
[0055] 3. Preparation of agar plates
[0056] Prepare a 2% agarose solution with distilled water, heat it in a microwave oven to dissolve, pour 15 to 20 mL of agarose solution into a 90 mm petri dish to condense for later use.
[0057] 4. Injection of colchicine
[0058] Microinjection of colchicine into flounder (Paralichthys olivaceus) larvae
[0059] (1) ...
Embodiment 2
[0077] The method for microinjecting colchicine to inhibit the movement of the left eye of Sole oleracea can be operated according to the following steps:
[0078] 1. Preparation of colchicine
[0079] Dilute the colchicine into a 5 mg / ml stock solution of colchicine with sterile 0.75% sodium chloride solution to prepare a 50 μg / ml colchicine solution.
[0080] 2. Preparation of anesthetics
[0081] Use freshly aerated seawater with a salinity of 32‰ and a temperature of 18°C to prepare a 0.1 mg / mL solution of ethyl 3-aminobenzoate methanesulfonate (tricaine methanesulfonate, MS-222), and prepare it immediately before use.
[0082] 3. Preparation of agar plates
[0083] Prepare a 2% agarose solution with distilled water, heat it in a microwave oven to dissolve, pour 15 to 20 mL of agarose solution into a 90 mm petri dish to condense for later use.
[0084] 4. Injection of colchicine
[0085] Microinjection of colchicine into Solea solea larvae
[0086] (1) Sole was rais...
Embodiment 3
[0099] The method of microinjecting colchicine to inhibit the movement of the right eye of tongue sole can be operated according to the following steps:
[0100] 1. Preparation of colchicine
[0101] Dilute the colchicine into a 5 mg / ml stock solution of colchicine with sterile 0.75% sodium chloride solution to prepare a 50 μg / ml colchicine solution.
[0102] 2. Preparation of anesthetics
[0103] Use freshly aerated seawater with a salinity of 32‰ and a temperature of 22°C to prepare a 0.1mg / mL solution of ethyl 3-aminobenzoate methanesulfonate (tricaine methanesulfonate, MS-222), and prepare it immediately before use.
[0104] 3. Preparation of agar plates
[0105] Prepare a 2% agarose solution with distilled water, heat it in a microwave oven to dissolve, pour 15 to 20 mL of agarose solution into a 90 mm petri dish to condense for later use.
[0106] 4. Injection of colchicine
[0107] Microinjection of colchicine into Cynoglossus semilaevis larvae
[0108] (1) Semi-sm...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com