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A kit for detecting Marfan syndrome and its preparation method

A kit and syndrome technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to achieve timely treatment or treatment, high accuracy of results, and high-throughput operation

Inactive Publication Date: 2011-12-14
SECOND AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the method of effectively preparing the kit both at home and abroad.

Method used

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  • A kit for detecting Marfan syndrome and its preparation method
  • A kit for detecting Marfan syndrome and its preparation method
  • A kit for detecting Marfan syndrome and its preparation method

Examples

Experimental program
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Embodiment Construction

[0050] The following specific examples are used to illustrate the present invention, but do not limit the scope of the present invention.

[0051] The following reagents were used in this example:

[0052] 1. Fetal genomic DNA extraction: Universal Genomic DNA Extraction Kit (provided by TaKaRa Bioengineering (Dalian) Co., Ltd. (TaKaRa), product number: DV811A).

[0053] 2. PCR reagents: primers (synthesized by Shanghai Yingjun Bioengineering Co., Ltd.): Contains six pairs of amplification primers (see the sequence listing for the sequence) to amplify the sequence to be tested; gold medal DNA polymerase (product of Applied Biosystem, USA) 10xPCR buffer (product of Applied Biosystem, U.S.); magnesium chloride (product of Applied Biosystem, U.S.); molecular biology grade water (product of Invitrogen, U.S.); 96-well PCR plate (product of Corning, U.S.).

[0054] 3. Cleaning of PCR products: clean enzyme for single base extension reaction (product of GE Healthcare in the United S...

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Abstract

The present invention relates to a method for prenatal diagnosis of Marfan syndrome, in particular to a method for prenatal diagnosis of Marfan syndrome using multiple polymerase chain reaction (PCR) and single base extension reaction, and also relates to a method for prenatal diagnosis of Marfan syndrome A kit for the diagnostic method of the present invention. The invention provides a kit for detecting Marfan syndrome with simple operation, simple equipment and high result accuracy and a method for preparing the kit. The technical solution is: a kit for detecting Marfan syndrome, which is characterized in that it includes specific primers, multiplex PCR reaction amplification liquid, cleaning liquid, single base extension reaction liquid, and the specific primers include each Six pairs or more than six pairs of multiple PCR amplification primers containing 18-25 bases and mutation detection primers containing 40-45 bases matching the detection site, wherein the mutation detection primers consist of the address sequence at the 5' end and The mutation detection region at the 3' end.

Description

technical field [0001] The present invention relates to a method for prenatal diagnosis of Marfan syndrome, in particular to a method for prenatal diagnosis of Marfan syndrome using multiple polymerase chain reaction (PCR) and single base extension reaction, and also relates to a Kits for use in the diagnostic methods of the present invention. Background technique [0002] Marfan syndrome (MFS) is one of the most common hereditary connective tissue diseases, mainly involving the bones, eyes and cardiovascular system. Its clinical features are: body abnormalities, such as slender and tall, slender limbs and fingers (toes), known as spider-like fingers (toes); eye lens dislocation; joint relaxation; heart and large vessel diseases, such as aortic root and Ascending aortic aneurysm-like dilation and acute or chronic aortic intimal rupture, forming dissecting aneurysms. In addition, there are a series of syndromes such as dural dilatation, high palate arch, funnel chest or chi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 孙朝晖金冲飞姚克
Owner SECOND AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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